Review



scfv antibody against murine notch3  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    R&D Systems scfv antibody against murine notch3
    Receptor specific and dose dependent inhibition of Notch signalling between cells. Luciferase based co-culture assays were conducted with cells expressing the ligand Jagged1 and cells expressing different full-length Notch receptors (HEK-Notch1, HEK-Notch2 and <t>HEK-Notch3).</t> (A) Samples tested were anti-Notch1 antibody N1_E6, anti-Notch2 antibodies N2_B6 and N2_B9, the anti-human <t>Notch3</t> antibody N3_mAb. Controls are non-activated, Jag1(-), and untreated, Jag1(+), cultures. The ratio of Firefly/Renilla luciferase activity is expressed relative to that of a control antibody (Shc1). (B) Dose response curves were generated by titration of N1_E6 on HEK-Notch1 and antibodies N2_B6 and N2_B9 on HEK-Notch2. The relative luciferase activity in non-activated cells were used to reflect 100% inhibition and the standard deviations were calculated to be 100 ± 6,65% for HEK-Notch1 (D) and 100 ± 2,51% for HEK-Notch2.
    Scfv Antibody Against Murine Notch3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scfv antibody against murine notch3/product/R&D Systems
    Average 90 stars, based on 13 article reviews
    scfv antibody against murine notch3 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells"

    Article Title: Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells

    Journal: Methods (San Diego, Calif.)

    doi: 10.1016/j.ymeth.2012.07.008

    Receptor specific and dose dependent inhibition of Notch signalling between cells. Luciferase based co-culture assays were conducted with cells expressing the ligand Jagged1 and cells expressing different full-length Notch receptors (HEK-Notch1, HEK-Notch2 and HEK-Notch3). (A) Samples tested were anti-Notch1 antibody N1_E6, anti-Notch2 antibodies N2_B6 and N2_B9, the anti-human Notch3 antibody N3_mAb. Controls are non-activated, Jag1(-), and untreated, Jag1(+), cultures. The ratio of Firefly/Renilla luciferase activity is expressed relative to that of a control antibody (Shc1). (B) Dose response curves were generated by titration of N1_E6 on HEK-Notch1 and antibodies N2_B6 and N2_B9 on HEK-Notch2. The relative luciferase activity in non-activated cells were used to reflect 100% inhibition and the standard deviations were calculated to be 100 ± 6,65% for HEK-Notch1 (D) and 100 ± 2,51% for HEK-Notch2.
    Figure Legend Snippet: Receptor specific and dose dependent inhibition of Notch signalling between cells. Luciferase based co-culture assays were conducted with cells expressing the ligand Jagged1 and cells expressing different full-length Notch receptors (HEK-Notch1, HEK-Notch2 and HEK-Notch3). (A) Samples tested were anti-Notch1 antibody N1_E6, anti-Notch2 antibodies N2_B6 and N2_B9, the anti-human Notch3 antibody N3_mAb. Controls are non-activated, Jag1(-), and untreated, Jag1(+), cultures. The ratio of Firefly/Renilla luciferase activity is expressed relative to that of a control antibody (Shc1). (B) Dose response curves were generated by titration of N1_E6 on HEK-Notch1 and antibodies N2_B6 and N2_B9 on HEK-Notch2. The relative luciferase activity in non-activated cells were used to reflect 100% inhibition and the standard deviations were calculated to be 100 ± 6,65% for HEK-Notch1 (D) and 100 ± 2,51% for HEK-Notch2.

    Techniques Used: Inhibition, Luciferase, Co-Culture Assay, Expressing, Activity Assay, Control, Generated, Titration

    Antibody-mediated inhibition of endogenous mouse and human Notch receptor signalling in neural stem cells. qRT-PCR was used to analyse the relative mRNA levels of Notch pathway genes in neural stem cell systems of both mouse and human origin. (A) Mouse NS cells express several Notch receptors and ligands. (B) Anti-Notch antibodies caused down-regulation of Notch dependent Hes5 expression in mouse NS cells relative to control antibody (Control ab). Inhibition of Hes5 with anti-NRR1 (N1) and anti-NRR2 (N2) antibodies are additive and co-incubation reduces Hes5 to the same extent as DAPT. (C) Notch receptors 1–3 and the ligands JAGGED1 and DLL1 and DLL3 are expressed in human NES cells (AF22). (D) Treating human NES cells with blocking antibodies targeting NRR1 (N1), NRR2 (N2) or NRR3 (N3) reduces HES5 expression. Co-incubation of anti-Notch1 with either anti-Notch2 or anti-Notch3 antibodies reduces HES5 expression to the same extent as inhibition with DAPT. Bars show relative mRNA levels as values normalised to GAPDH based on two separate reactions (A and C). Bars in figure B and D show fold change compared to DMSO control experiments.
    Figure Legend Snippet: Antibody-mediated inhibition of endogenous mouse and human Notch receptor signalling in neural stem cells. qRT-PCR was used to analyse the relative mRNA levels of Notch pathway genes in neural stem cell systems of both mouse and human origin. (A) Mouse NS cells express several Notch receptors and ligands. (B) Anti-Notch antibodies caused down-regulation of Notch dependent Hes5 expression in mouse NS cells relative to control antibody (Control ab). Inhibition of Hes5 with anti-NRR1 (N1) and anti-NRR2 (N2) antibodies are additive and co-incubation reduces Hes5 to the same extent as DAPT. (C) Notch receptors 1–3 and the ligands JAGGED1 and DLL1 and DLL3 are expressed in human NES cells (AF22). (D) Treating human NES cells with blocking antibodies targeting NRR1 (N1), NRR2 (N2) or NRR3 (N3) reduces HES5 expression. Co-incubation of anti-Notch1 with either anti-Notch2 or anti-Notch3 antibodies reduces HES5 expression to the same extent as inhibition with DAPT. Bars show relative mRNA levels as values normalised to GAPDH based on two separate reactions (A and C). Bars in figure B and D show fold change compared to DMSO control experiments.

    Techniques Used: Inhibition, Quantitative RT-PCR, Expressing, Control, Incubation, Blocking Assay



    Similar Products

    scfv antibody against murine notch3  (R&D Systems)
    90
    R&D Systems scfv antibody against murine notch3
    Receptor specific and dose dependent inhibition of Notch signalling between cells. Luciferase based co-culture assays were conducted with cells expressing the ligand Jagged1 and cells expressing different full-length Notch receptors (HEK-Notch1, HEK-Notch2 and <t>HEK-Notch3).</t> (A) Samples tested were anti-Notch1 antibody N1_E6, anti-Notch2 antibodies N2_B6 and N2_B9, the anti-human <t>Notch3</t> antibody N3_mAb. Controls are non-activated, Jag1(-), and untreated, Jag1(+), cultures. The ratio of Firefly/Renilla luciferase activity is expressed relative to that of a control antibody (Shc1). (B) Dose response curves were generated by titration of N1_E6 on HEK-Notch1 and antibodies N2_B6 and N2_B9 on HEK-Notch2. The relative luciferase activity in non-activated cells were used to reflect 100% inhibition and the standard deviations were calculated to be 100 ± 6,65% for HEK-Notch1 (D) and 100 ± 2,51% for HEK-Notch2.
    Scfv Antibody Against Murine Notch3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scfv antibody against murine notch3/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    scfv antibody against murine notch3 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Receptor specific and dose dependent inhibition of Notch signalling between cells. Luciferase based co-culture assays were conducted with cells expressing the ligand Jagged1 and cells expressing different full-length Notch receptors (HEK-Notch1, HEK-Notch2 and HEK-Notch3). (A) Samples tested were anti-Notch1 antibody N1_E6, anti-Notch2 antibodies N2_B6 and N2_B9, the anti-human Notch3 antibody N3_mAb. Controls are non-activated, Jag1(-), and untreated, Jag1(+), cultures. The ratio of Firefly/Renilla luciferase activity is expressed relative to that of a control antibody (Shc1). (B) Dose response curves were generated by titration of N1_E6 on HEK-Notch1 and antibodies N2_B6 and N2_B9 on HEK-Notch2. The relative luciferase activity in non-activated cells were used to reflect 100% inhibition and the standard deviations were calculated to be 100 ± 6,65% for HEK-Notch1 (D) and 100 ± 2,51% for HEK-Notch2.

    Journal: Methods (San Diego, Calif.)

    Article Title: Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells

    doi: 10.1016/j.ymeth.2012.07.008

    Figure Lengend Snippet: Receptor specific and dose dependent inhibition of Notch signalling between cells. Luciferase based co-culture assays were conducted with cells expressing the ligand Jagged1 and cells expressing different full-length Notch receptors (HEK-Notch1, HEK-Notch2 and HEK-Notch3). (A) Samples tested were anti-Notch1 antibody N1_E6, anti-Notch2 antibodies N2_B6 and N2_B9, the anti-human Notch3 antibody N3_mAb. Controls are non-activated, Jag1(-), and untreated, Jag1(+), cultures. The ratio of Firefly/Renilla luciferase activity is expressed relative to that of a control antibody (Shc1). (B) Dose response curves were generated by titration of N1_E6 on HEK-Notch1 and antibodies N2_B6 and N2_B9 on HEK-Notch2. The relative luciferase activity in non-activated cells were used to reflect 100% inhibition and the standard deviations were calculated to be 100 ± 6,65% for HEK-Notch1 (D) and 100 ± 2,51% for HEK-Notch2.

    Article Snippet: Along with the blocking antibodies, an anti-Notch3 antibody, N3(E10), previously selected as a scFv antibody against murine Notch3 (R&D systems) (unpublished), was also converted to scFv-Fc for use in flow cytometry ( ).

    Techniques: Inhibition, Luciferase, Co-Culture Assay, Expressing, Activity Assay, Control, Generated, Titration

    Antibody-mediated inhibition of endogenous mouse and human Notch receptor signalling in neural stem cells. qRT-PCR was used to analyse the relative mRNA levels of Notch pathway genes in neural stem cell systems of both mouse and human origin. (A) Mouse NS cells express several Notch receptors and ligands. (B) Anti-Notch antibodies caused down-regulation of Notch dependent Hes5 expression in mouse NS cells relative to control antibody (Control ab). Inhibition of Hes5 with anti-NRR1 (N1) and anti-NRR2 (N2) antibodies are additive and co-incubation reduces Hes5 to the same extent as DAPT. (C) Notch receptors 1–3 and the ligands JAGGED1 and DLL1 and DLL3 are expressed in human NES cells (AF22). (D) Treating human NES cells with blocking antibodies targeting NRR1 (N1), NRR2 (N2) or NRR3 (N3) reduces HES5 expression. Co-incubation of anti-Notch1 with either anti-Notch2 or anti-Notch3 antibodies reduces HES5 expression to the same extent as inhibition with DAPT. Bars show relative mRNA levels as values normalised to GAPDH based on two separate reactions (A and C). Bars in figure B and D show fold change compared to DMSO control experiments.

    Journal: Methods (San Diego, Calif.)

    Article Title: Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells

    doi: 10.1016/j.ymeth.2012.07.008

    Figure Lengend Snippet: Antibody-mediated inhibition of endogenous mouse and human Notch receptor signalling in neural stem cells. qRT-PCR was used to analyse the relative mRNA levels of Notch pathway genes in neural stem cell systems of both mouse and human origin. (A) Mouse NS cells express several Notch receptors and ligands. (B) Anti-Notch antibodies caused down-regulation of Notch dependent Hes5 expression in mouse NS cells relative to control antibody (Control ab). Inhibition of Hes5 with anti-NRR1 (N1) and anti-NRR2 (N2) antibodies are additive and co-incubation reduces Hes5 to the same extent as DAPT. (C) Notch receptors 1–3 and the ligands JAGGED1 and DLL1 and DLL3 are expressed in human NES cells (AF22). (D) Treating human NES cells with blocking antibodies targeting NRR1 (N1), NRR2 (N2) or NRR3 (N3) reduces HES5 expression. Co-incubation of anti-Notch1 with either anti-Notch2 or anti-Notch3 antibodies reduces HES5 expression to the same extent as inhibition with DAPT. Bars show relative mRNA levels as values normalised to GAPDH based on two separate reactions (A and C). Bars in figure B and D show fold change compared to DMSO control experiments.

    Article Snippet: Along with the blocking antibodies, an anti-Notch3 antibody, N3(E10), previously selected as a scFv antibody against murine Notch3 (R&D systems) (unpublished), was also converted to scFv-Fc for use in flow cytometry ( ).

    Techniques: Inhibition, Quantitative RT-PCR, Expressing, Control, Incubation, Blocking Assay